Pancreatic Cancer Research Today is a free monthly online journal that collates and summarizes the latest research about Pancreatic Cancer, including details on symptoms, causes, treatment, information.

Plasma proteomic analysis of pancreatic cancer by 2-dimensional gel electrophoresis.

Deng R, Lu Z, Chen Y, Zhou L, Lu X

Department of Gastroenterology, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, PR China.

OBJECTIVE: Late diagnosis is the major reason for poor prognosis of pancreatic cancer (PC). Developing new biomarkers of early stage detection is critical. METHODS: Proteomic analysis with 2-dimensional gel electrophoresis was used to identify differentially expressed proteins in plasma of PC patients. The 2-dimensional gel electrophoresis plasma protein profiles of 11 PC patients (preoperative and postoperative) were compared with those of 10 patients with chronic pancreatitis (CP) and 11 healthy controls. RESULTS: Five proteins were found to be constantly changed. Haptoglobin (Hp) beta chain and leucine-rich alpha2 glycoprotein up-regulated slightly in PC plasma. Pancreatic cancer had a higher frequency of Hp2-2 phenotype but lacked Hp1-1 phenotype. Hemoglobin was increased significantly in plasma samples of PC and CP. Alpha1 antitrypsin gradually increased its expression level in healthy control, PC, and CP. Immunoglobin J chain was elevated in CP plasma samples. Haptoglobin, alpha1 antitrypsin, and leucine-rich alpha2-glycoprotein were all greatly elevated after tumor resection in PC patients. CONCLUSIONS: Proteomic analysis can simultaneously detect changes of multiproteins in plasma of PC, but detected proteins are abundant and common plasma proteins and their diagnostic value may be limited.

Published 6 April 2007 in Pancreas, 34(3): 310-7.
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